- The Chitwan valley is characterized by tropical to subtropical forest. 70% of park vegetation is predominantly Sal ( Shorea robusta ) forest, a moist deciduous climax vegetation type of the Terai region. The remaining vegetation types include grassland (20%), riverine forest (7%) and Sal with Chirpine ( Pinus roxburghii ) (3%), the latter occurring at the top of the Churia range. The riverine forests mainly consists of khair, sissoo and simal. The simal is with spiny bark when young and develops buttress at the bottom in older stage. The grasslands form a diverse and complex community with over 50 species. The Sacchrum species, often called elephant-grass can reach 8 m in height. The shorter grasses such as Imperata is useful for thatch roofs.
- There are more than 43 species of mammals in the park. The park is specially renowned for the protection of the endangered one-horned rhinoceros, tiger, gharial crocodile along with many other common species of wild animals. The estimated population of endangered species of animals such as gaur, wild elephant, four horned antelope, striped hyena, pangolin, gangetic dolphin, monitor lizard and python, etc.
- Some of the other animals found in the park are samber, chital, hog deer, barking deer, sloth bear, common leopard, ratel, palm civet, wild dog, langur, rhesus monkey, etc.
- There are over 450 species of birds in the park. Among the endangered birds found in the park are Bengal florican, giant hornbill, lesser florican, black stork and white stork, Few of the common birds seen are peafowl, red jungle fowl, and different species of egrets, herons, kingfishers, flycatchers and woodpeckers. The best time for bird watching is March and December.
- More than 45 species of amphibians and reptiles occur in the park and some of which are marsh mugger crocodile, cobra, green pit viper and various species of frogs and tortoises.
- The park is actively engaged in the scientific studies of several species of wild flora and fauna.
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